Key result
Digital video imaging of resting rat cardiac myocytes revealed a cytosolic free Ca2+ concentration of 90 +/- 6 nM with no subcellular gradients, though heterogeneity occurred during contraction.
Population
Single isolated Ca2+-tolerant rat ventricular myocytes in primary culture
Design
Preclinical
Authors
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Informs basic models of myocyte Ca2+ handling; leaves open translation to human or in vivo cardiovascular physiology.
Digital video imaging of fura-2 fluorescence reveals that resting rat ventricular myocytes have a uniform cytosolic free calcium concentration of ~90 nM, with subcellular heterogeneity appearing during spontaneous contractions.
Cheung et al. (1989) studied Isolated rat ventricular myocytes. Digital video imaging of intracellular fura-2 fluorescence was evaluated on Cytosolic free Ca2+ concentration ([Ca2+]i). Digital video imaging of resting rat cardiac myocytes revealed a cytosolic free Ca2+ concentration of 90 +/- 6 nM with no subcellular gradients, though heterogeneity occurred during contraction.