Key result
Mitochondrial free Ca2+ concentration in unstimulated rat cardiac myocytes is low (<100 nM) but increases up to 600 nM during high-frequency pacing (4 Hz) with norepinephrine.
Population
living single rat cardiac myocytes
Design
Preclinical
Authors
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Allows continuous [Ca2+]m monitoring in living myocytes; leaves open validation for physiological studies.
A novel technique using indo-1 allows continuous measurement of mitochondrial free Ca2+ in single living rat cardiac myocytes, revealing that [Ca2+]m increases with pacing frequency and norepinephrine, consistent with a signal to increase oxidative phosphorylation.
Miyata et al. (1991) studied this question. Measurement of mitochondrial free Ca2+ using indo-1 and Mn2+ quenching was evaluated on Mitochondrial free Ca2+ concentration ([Ca2+]m). Mitochondrial free Ca2+ concentration in unstimulated rat cardiac myocytes is low (<100 nM) but increases up to 600 nM during high-frequency pacing (4 Hz) with norepinephrine.
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