Efficient Coutilization of Methanol with Pentose Sugars by Engineered Corynebacterium glutamicum for Protocatechuic Acid Production

Key Points

• The aim is to efficiently utilize methanol and pentose sugars for protocatechuic acid production using engineered bacteria.
• Utilized xylose-containing hydrolysate from orange peels for PCA production
• Engineered strains of Corynebacterium glutamicum were assessed for high titer production
• Measured PCA yield to evaluate efficiency and sustainability.
• Achieved a protocatechuic acid titer of 10.61 g/L from methanol and pentose sugars
• Demonstrated first production of PCA by a methanol-essential strain
• Highlighted sustainable bioproduction options from food-waste feedstocks.