This research aims to understand how RYR complexes influence the interaction between the endoplasmic reticulum and lysosomes in the context of autophagy control.
Investigated the role of RYR:ATP6V0A1 complexes in cellular calcium signaling and autophagy dynamics.
Monitored changes in ER-lysosome contact sites using imaging techniques and biochemical assays.
Analyzed the effects of altered RYR activity on autophagic processes in various cell types.
Enhanced RYR activity significantly increased autophagy flux, indicated by higher LC3-II levels compared to controls (p<0.01).
ER-lysosome contact site integrity was correlated with autophagic efficiency, suggesting a direct interaction (p<0.05).
Inhibition of RYR led to reduced calcium levels in lysosomes and subsequent impairment of autophagy.