Abstract Background: Since the beginning of time, people have employed a variety of plants and animals to prevent and treat illnesses. Leeches produce physiologically and pharmacologically active tools into the wound when they are feeding. Bloodsuckers and infections becoming resistant to synthetic medications have increased the need for new, environmentally safe, and active control methods. Objectives: The aim of present study is to extract, synthesize, and characterize Hirudo medicinalis saliva. Materials and Methods: Twelve weeks starvation of Hirudo Medicinalis , leech saliva extraction done by feeding with phagostimulatory solution, leech then freezing for 15 min and remove from ice bag vomited saliva then squeezed to collect saliva, centrifuged at 4°C, 9000 rpm for 10 min. For synthesis Ag-NPs saliva, 0.1 g AgNo 3 was mixed with the 1 mL crude leech saliva at controlled conditions of darkness, absence of oxygen, and 25°C, Ag-NPs were kept in a tube covered with aluminum foil, and stored at 4°C. Ag-NPs characterization was done using a dynamic light scattering test and field emission scanning electron microscopy. Results: The starved leeches give saliva. Leech Ag-NPs size is 694.1 and zeta potential is −0.060 by Dynamic light scattering, square shape about 20–720 nm with an average value of 600 by field emission scanning electron microscopy. Conclusion: Saliva collection from starved and feeding leech with phagostimulatory solution and Ag-NPs saliva synthesized.
Witwit et al. (Thu,) studied this question.