What question did this study set out to answer?

This research aims to characterize the roles of four B-type cyclin genes in the budding yeast Saccharomyces cerevisiae, focusing on their timing and function during cell division.

July 1, 1992Molecular Biology of the CellOpen Access

Characterization of four B-type cyclin genes of the budding yeast Saccharomyces cerevisiae.

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This research aims to characterize the roles of four B-type cyclin genes in the budding yeast Saccharomyces cerevisiae, focusing on their timing and function during cell division.
Characterized complete sequences of CLB1, CLB2, CLB3, and CLB4 genes.
Constructed various deletion mutants to assess viability and interactions.
Utilized electron microscopy to study spindle pole body behavior during mitosis.
All single deletion mutants were viable, but certain multiple mutants were lethal, particularly involving the clb2 deletion.
Conditional clb mutants exhibited defects in mitosis, arresting with G2 DNA content and without a formed spindle.
Electron microscopy revealed duplicated but unsplit spindle pole bodies, indicating a role for Clb/Cdc28 in spindle formation.

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Implication

Experimental analysis characterizes B-type cyclin genes in yeast, revealing distinct roles in mitosis and spindle formation.

Cite This Study

Fitch et al. (1992) studied this question.

synapsesocial.com/papers/69fd8447f8347cff7f5d1a6a https://doi.org/10.1091/mbc.3.7.805

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Characterization of four B-type cyclin genes of the budding yeast Saccharomyces cerevisiae.

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Discussion

Implication

Cite This Study

Characterization of four B-type cyclin genes of the budding yeast Saccharomyces cerevisiae.

Key Points

Discussion

Implication

Cite This Study

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