Long-term Physiological Parameter Measurement in Awake Mice via Carotid Artery Radio Telemetry Implantation: OSAS Experimental Model

ABS TRACT This study presents a refined protocol for surgically implanting the DSI radio telemetry device into the carotid artery, enabling continuous monitoring of key physiological parameters, including blood pressure, heart rate, body temperature, and activity, in conscious, freely moving mice.The method offers a substantial advantage by eliminating the need for physical restraint or immobilization, which can often induce stress and introduce measurement artifacts during prolonged data collection.However, catheterizing small arteries remains technically demanding, particularly in juvenile and highly active mice.This protocol provides a detailed guide for the successful implantation of radio transmitters via carotid artery cannulation and includes examples of data acquisition during surgery and recovery.By following this optimized procedure, researchers can achieve reliable, long-term, and concurrent monitoring of physiological parameters, enhancing precision and consistency across multiple subjects.