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Shows antibody recognition of phosphorylated 53BP1, indicating potential for protein analysis.
The engineered Forkhead-associated Domain (FHA1), located at the N-terminus of the RAD53 protein of Saccharomyces cerevisiae, was shown in previous studies to specifically recognize the phosphorylated peptide of the p53-binding protein (53BP1). When the engineered FHA1 domain was expressed as a recombinant rabbit Fc-fusion protein, it still recognized the phosphorylated 53BP1 peptide in an ELISA. FHA1-Fc fusion proteins can thus be used as specific antibodies to identify the phosphorylation status of various proteins.
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Thota et al. (2026) studied this question.
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