Evidence supporting the first secondary chromosome in Actinobacteria as a hallmark of the Embleya genus.

Key Points

• The research aims to identify and characterize a secondary chromosome in the Embleya genus of Actinobacteria.
• Proposed EEC1 as a secondary chromosome based on genetic analysis.
• Characterization of replicon sizes within Actinobacteria.
• Identified the largest secondary replicon reported in bacteria to date.
• EEC1 is confirmed as a secondary chromosome, highlighting its significance in genetic diversity.