What question did this study set out to answer?

To determine how mammalian SIRT6 mediates DNA double-strand break repair and genome stability in cells exposed to oxidative stress.

June 17, 2011ScienceOpen Access

SIRT6 Promotes DNA Repair Under Stress by Activating PARP1

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Authors

ZMZhiyong MaoShanghai First Maternity and Infant Hospital CHChristopher HineCleveland Clinic XTXiao TianShandong University

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Implication

Experimental study demonstrates SIRT6 recruitment and PARP1 activation during DNA break repair in mammalian cells, indicating a key mechanism for maintaining genome stability under oxidative stress.

Key Points

To determine how mammalian SIRT6 mediates DNA double-strand break repair and genome stability in cells exposed to oxidative stress.
Assessed SIRT6 recruitment to DNA double-strand break sites in mammalian cells subjected to oxidative stress.
Evaluated double-strand break repair activity through both nonhomologous end joining and homologous recombination pathways.
Analyzed the physical interaction and enzymatic modification between SIRT6 and PARP1.
SIRT6 localizes to DNA double-strand break sites under oxidative stress and enhances repair through both nonhomologous end joining and homologous recombination.
SIRT6 physically interacts with PARP1 and mono-ADP-ribosylates PARP1 specifically at lysine residue 521.
Mono-ADP-ribosylation by SIRT6 stimulates PARP1 poly-ADP-ribosylase activity, driving enhanced double-strand break repair under oxidative stress conditions.

Cite This Study

Mao et al. (2011) studied this question.

synapsesocial.com/papers/69ff50fd4716aad0cc8549f0 https://doi.org/10.1126/science.1202723

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Implication

Experimental study demonstrates SIRT6 recruitment and PARP1 activation during DNA break repair in mammalian cells, indicating a key mechanism for maintaining genome stability under oxidative stress.

SIRT6 Promotes DNA Repair Under Stress by Activating PARP1

Discussion

Member takes

Implication

Key Points

Cite This Study

SIRT6 Promotes DNA Repair Under Stress by Activating PARP1

Discussion

Member takes

Implication

Key Points

Cite This Study

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