Diagnostic evaluation uncovers diverse parasitic protists in pigs, indicating that combining metabarcoding with real-time PCR enhances zoonotic pathogen surveillance.
. Our results illustrate how metabarcoding exemplifies a 'one-fits-many' approach to detecting intestinal single-celled parasites in feces supplemented with real-time PCR for selected parasites. Using metabarcoding with pathogen-specific assays may help detect emerging and previously underdetected pathogens and further elucidate the role of micro-eukaryotic parasites in human and animal health and disease.
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Stensvold et al. (2021) studied this question.
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