Molecular Classification of Endometrial Stromal Sarcomas Using RNA Sequencing Defines Nosological and Prognostic Subgroups with Different Natural History

Figures

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  Figure 1. Consensus hierarchical unsupervised clustering of expression profiles extracted from FFPE tumor samples. Consensus for k = 3 cluster is shown. Sample, tumor types and identified fusion genes are indicated. Single sample GSEA (Gene Set Enrichment Analysis) G2/M cell cycle score (CCS) for each samples is represented in a blue–red normalized scale.

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  Figure 2. Kaplan–Meier survival analysis of the retrospective series of primary diagnosed ESS stratified by the clusters (log-rank, p = 0.004; Wilcoxon); x-axis, time in months. The three subgroups display different gene signatures and different in silico cell cycle scores that significantly correlated with different overall survivals.

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  Figure 3. Hierarchical unsupervised clustering of the investigation cohort (n = 43) upon immune infiltrate population profiles. Cluster analyses generated one group with high (H) immune infiltrate and one group with low immune infiltrate subdivided in 3 slightly different subtypes: very low (VL), low (L) and intermediate (I). Clusters from Figure 1 and final diagnosis are also reported.