What question did this study set out to answer?

To determine whether pharmacological inhibition of long myosin light-chain kinase attenuates intestinal barrier breakdown and inflammatory responses following combined binge ethanol exposure and burn trauma.

July 13, 2012AJP Gastrointestinal and Liver Physiology

Inhibition of long myosin light-chain kinase activation alleviates intestinal damage after binge ethanol exposure and burn injury

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Authors

AZAnita ZahsLoyola University Medical Center MBMelanie D. BirdAmerican Association of Clinical Endocrinologists LRLuis RamírezHospital Clínic de Barcelona

Discussion

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Implication

Animal study reveals that inhibiting myosin light-chain kinase prevents gut barrier disruption and inflammation in mice after alcohol exposure and burns, highlighting a potential therapeutic target.

Key Points

To determine whether pharmacological inhibition of long myosin light-chain kinase attenuates intestinal barrier breakdown and inflammatory responses following combined binge ethanol exposure and burn trauma.
Mice received vehicle or a single binge ethanol exposure followed by a sham procedure or dorsal scald burn injury.
A subset of injured mice received a membrane permeant inhibitor of myosin light-chain kinase (PIK) postinjury.
Evaluations at 6 and 24 hours postinjury assessed intestinal histomorphology, tight junction protein localization (ZO-1 and occludin), ileal cytokine levels (IL-1β, IL-6), and bacterial translocation.
Combined binge ethanol and burn trauma aggravated ileal tissue injury, elevated IL-1β and IL-6 concentrations, and increased bacterial translocation compared to either insult alone.
Postinjury administration of PIK prevented the increases in tissue damage, proinflammatory cytokine production, and bacterial translocation.
Ethanol exposure coupled with burn injury disrupted zonula occludens protein-1 and occludin localization at epithelial tight junctions, whereas PIK treatment preserved junctional complex integrity.

Cite This Study

Zahs et al. (2012) studied this question.

synapsesocial.com/papers/69ff7a636be84a7ac8853ffe https://doi.org/10.1152/ajpgi.00157.2012

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Implication

Animal study reveals that inhibiting myosin light-chain kinase prevents gut barrier disruption and inflammation in mice after alcohol exposure and burns, highlighting a potential therapeutic target.

Inhibition of long myosin light-chain kinase activation alleviates intestinal damage after binge ethanol exposure and burn injury

Discussion

Member takes

Implication

Key Points

Cite This Study

Inhibition of long myosin light-chain kinase activation alleviates intestinal damage after binge ethanol exposure and burn injury

Discussion

Member takes

Implication

Key Points

Cite This Study

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