What question did this study set out to answer?

To determine whether monocyte chemotactic protein-1 (MCP-1)-induced angiogenesis is mediated through the novel transcription factor MCPIP.

March 26, 2008Journal of International Crisis and Risk Communication ResearchOpen Access

Monocyte Chemotactic Protein (MCP)-1 Promotes Angiogenesis via a Novel Transcription Factor, MCP-1-induced Protein (MCPIP)

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Authors

JNJianli NiuMemorial Healthcare System AAAsim AzferWestern General Hospital OZOlga ZhelyabovskaUniversity of Central Florida

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Implication

In vitro experimental study demonstrates that MCP-1 drives capillary-like tube formation through MCPIP in human endothelial cells, indicating a transcriptional pathway for angiogenesis.

Key Points

To determine whether monocyte chemotactic protein-1 (MCP-1)-induced angiogenesis is mediated through the novel transcription factor MCPIP.
Treated human umbilical vein endothelial cells (HUVECs) with MCP-1 and analyzed MCPIP expression and capillary-like tube formation.
Performed siRNA-mediated knockdown and vector-based overexpression of MCPIP, cadherin 12 (cdh12), and cadherin 19 (cdh19) in HUVECs.
Identified direct genomic binding targets of MCPIP using chromatin immunoprecipitation (ChIP) assays.
MCP-1 treatment induced MCPIP expression and capillary-like tube formation, whereas siRNA knockdown of MCPIP suppressed tube formation along with VEGF and HIF-1alpha expression.
MCPIP overexpression directly upregulated angiogenesis-related genes and induced capillary-like tube formation in endothelial cells.
Chromatin immunoprecipitation demonstrated that cdh12 and cdh19 are direct targets of MCPIP, and silencing either cadherin significantly inhibited MCPIP-mediated tube formation.

Cite This Study

Niu et al. (2008) studied this question.

synapsesocial.com/papers/69ff7e01e4618ba4162d7e97 https://doi.org/10.1074/jbc.m802139200

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Implication

In vitro experimental study demonstrates that MCP-1 drives capillary-like tube formation through MCPIP in human endothelial cells, indicating a transcriptional pathway for angiogenesis.

Monocyte Chemotactic Protein (MCP)-1 Promotes Angiogenesis via a Novel Transcription Factor, MCP-1-induced Protein (MCPIP)

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Implication

Key Points

Cite This Study

Monocyte Chemotactic Protein (MCP)-1 Promotes Angiogenesis via a Novel Transcription Factor, MCP-1-induced Protein (MCPIP)

Discussion

Member takes

Implication

Key Points

Cite This Study

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