What question did this study set out to answer?

To identify the viral genetic determinants responsible for enabling tomato mosaic virus to overcome the Tm-2(2) resistance gene in tomatoes.

November 1, 1993Journal of VirologyOpen Access

Two amino acid substitutions in the tomato mosaic virus 30-kilodalton movement protein confer the ability to overcome the Tm-2(2) resistance gene in the tomato

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Authors

HWHans WeberLeibniz Institute of Plant Genetics and Crop Plant Research SSSabine SchultzeLudwig-Maximilians-Universität München APArtur J.P. PfitznerUniversity of Hohenheim

Discussion

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Implication

Molecular genetic analysis reveals that two amino acid substitutions in viral movement protein overcome host plant resistance in tomatoes, indicating specific recognition-driven immunity.

Key Points

To identify the viral genetic determinants responsible for enabling tomato mosaic virus to overcome the Tm-2(2) resistance gene in tomatoes.
Engineered chimeric full-length viral cDNA clones under the cauliflower mosaic virus 35S promoter combining wild-type ToMV and resistance-breaking ToMV-2(2) sequences.
Identified four candidate amino acid mutations via sequence analysis and systematically introduced combinations of these mutations into wild-type viral genomes.
Inoculated Tm-2(2) tomato plants with mutant viral cDNA constructs to evaluate their replication and resistance-breaking capabilities.
Sequencing identified four amino acid differences between the 30-kDa movement proteins: Lys-130 to Glu, Gly-184 to Glu, Ser-238 to Arg, and Lys-244 to Glu.
Only the double mutant containing Arg-238 and Glu-244 in the carboxy-terminal region of the movement protein successfully overcame Tm-2(2) resistance and multiplied in resistant tomato plants.

Cite This Study

Weber et al. (1993) studied this question.

synapsesocial.com/papers/69ff91b9da5c1eb07f2d7e2e https://doi.org/10.1128/jvi.67.11.6432-6438.1993

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November 1, 1993Journal of VirologyOpen Access

Two amino acid substitutions in the tomato mosaic virus 30-kilodalton movement protein confer the ability to overcome the Tm-2(2) resistance gene in the tomato

View Full Paper

Ask AI

Bookmark

Authors

HWHans WeberLeibniz Institute of Plant Genetics and Crop Plant Research SSSabine SchultzeLudwig-Maximilians-Universität München APArtur J.P. PfitznerUniversity of Hohenheim

Discussion

Member takes

Implication

Molecular genetic analysis reveals that two amino acid substitutions in viral movement protein overcome host plant resistance in tomatoes, indicating specific recognition-driven immunity.

Key Points

To identify the viral genetic determinants responsible for enabling tomato mosaic virus to overcome the Tm-2(2) resistance gene in tomatoes.
Engineered chimeric full-length viral cDNA clones under the cauliflower mosaic virus 35S promoter combining wild-type ToMV and resistance-breaking ToMV-2(2) sequences.
Identified four candidate amino acid mutations via sequence analysis and systematically introduced combinations of these mutations into wild-type viral genomes.
Inoculated Tm-2(2) tomato plants with mutant viral cDNA constructs to evaluate their replication and resistance-breaking capabilities.
Sequencing identified four amino acid differences between the 30-kDa movement proteins: Lys-130 to Glu, Gly-184 to Glu, Ser-238 to Arg, and Lys-244 to Glu.
Only the double mutant containing Arg-238 and Glu-244 in the carboxy-terminal region of the movement protein successfully overcame Tm-2(2) resistance and multiplied in resistant tomato plants.

Cite This Study

Weber et al. (1993) studied this question.

synapsesocial.com/papers/69ff91b9da5c1eb07f2d7e2e https://doi.org/10.1128/jvi.67.11.6432-6438.1993

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