Can an aptamer-based electrochemical sensor detect TNF-α in whole human blood?
An aptamer-based electrochemical sensor can successfully detect TNF-α in whole human blood, offering potential for rapid point-of-care diagnosis.
Electrochemical aptasensors can detect disease markers such as cytokines to provide point-of-care diagnosis that is low-cost, rapid, specific and sensitive. Herein, we describe the development of an aptamer-based electrochemical sensor for detection and analysis of tumor necrosis factor-alpha (TNF-α) - a key inflammatory cytokine - in whole human blood. When testing spiked blood, a TNF-α detection limit of 58 pM (10 ng mL(-1)) and a linear range of 6 nM (100 ng mL(-1)) could be achieved. Furthermore, detection of TNF-α in mitogen stimulated whole blood was demonstrated.
Liu et al. (Tue,) studied this question.