What question did this study set out to answer?

To investigate the effects of specific advanced glycation end-products on human mesenchymal stem cell survival, proliferation, and multilineage differentiation into fat, cartilage, and bone.

September 1, 2005Journal of Bone and Mineral ResearchOpen Access

Advanced Glycation End-Products Attenuate Human Mesenchymal Stem Cells and Prevent Cognate Differentiation Into Adipose Tissue, Cartilage, and Bone

View Full Paper

Ask AI

Bookmark

Authors

SKShinichiro KumeOita University SKSeiya KatoOsaka Gakuin University SYSho‐ichi YamagishiGeneral / Preventive / Lipids

Discussion

Member takes

Implication

In vitro study demonstrates that advanced glycation end-products impair human mesenchymal stem cell differentiation, indicating a driver of defective musculoskeletal repair in diabetes and aging.

Key Points

To investigate the effects of specific advanced glycation end-products on human mesenchymal stem cell survival, proliferation, and multilineage differentiation into fat, cartilage, and bone.
Cultured human mesenchymal stem cells with distinct advanced glycation end-product subtypes (AGE-1, AGE-2, and AGE-3) or unmodified bovine serum albumin control.
Assayed cell proliferation via BrdU incorporation, apoptosis, reactive oxygen species generation, and RAGE expression with and without a neutralizing anti-RAGE antiserum.
Assessed adipogenic, chondrogenic, and osteogenic differentiation using lineage-specific morphological staining and biochemical markers.
AGE-2 and AGE-3, but not unglycated albumin or AGE-1, significantly reduced viable cell numbers and BrdU incorporation while accelerating intracellular ROS generation and apoptosis.
AGE-2 and AGE-3 induced RAGE expression, inhibited adipogenesis and chondrogenesis, and impaired functional bone mineralization despite increasing alkaline phosphatase activity and calcium content.
Neutralizing antiserum directed against RAGE partially rescued cells from AGE-induced cytotoxicity and differentiation defects.

Cite This Study

Kume et al. (2005) studied this question.

synapsesocial.com/papers/69ffb0a6ef8139f8ff776ceb https://doi.org/10.1359/jbmr.050514

View Full Paper

Ask AI

Bookmark

Implication

In vitro study demonstrates that advanced glycation end-products impair human mesenchymal stem cell differentiation, indicating a driver of defective musculoskeletal repair in diabetes and aging.

Advanced Glycation End-Products Attenuate Human Mesenchymal Stem Cells and Prevent Cognate Differentiation Into Adipose Tissue, Cartilage, and Bone

Discussion

Member takes

Implication

Key Points

Cite This Study

Advanced Glycation End-Products Attenuate Human Mesenchymal Stem Cells and Prevent Cognate Differentiation Into Adipose Tissue, Cartilage, and Bone

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider