VAE-MOTION provides a novel deep learning approach to accurately extract cardiomyocyte contractility parameters and restore video quality from time-lapse microscopy, improving preclinical drug evaluation.
Deep learning has proven to be one of the most effective methods in analyzing biological images to extract parameters fundamental for studying physiological functions and pathological conditions. In particular, when coupled with time-lapse microscopy (TLM), deep learning proves particularly effective in studying behaviors involving temporal dynamics. However, TLM videos are often affected by experimental noise and setup limitations, which can lead to inaccurate and poorly reproducible results. Taking advantage of the variational and generative capabilities of Variational Autoencoders (VAEs), we propose VAE-MOTION, a deep learning-based model for the analysis of cardiac contractile dynamics. By incorporating a temporal encoder into its architecture, our model allows the restoration of video quality by removing noise or increasing resolution, while simultaneously extracting accurate contraction-related signals from the latent space. The generation of synthetic videos allowed extensive training of VAE-MOTION, which subsequently validated on real videos from two different cardiac tissue models: 2D monolayers and 3D microtissues. VAE-MOTION was compared to two gold-standard methods in extracting contraction parameters relevant to drug efficacy or toxicity studies, demonstrating its potential for analyzing temporal dynamics in a given phenomenon or process.
Curci et al. (Tue,) studied this question.