What question did this study set out to answer?

To evaluate the efficiency, longevity, and immunogenicity of recombinant adeno-associated virus (rAAV) vectors for in vivo gene transfer into mammalian muscle tissue.

November 1, 1996Journal of VirologyOpen Access

Efficient long-term gene transfer into muscle tissue of immunocompetent mice by adeno-associated virus vector

View Full Paper

Ask AI

Bookmark

Why the study?

Does recombinant adeno-associated virus vector (rAAV) achieve long-term gene transduction in muscle tissue of immunocompetent mice?

Population

Immunocompetent mice

Design

Preclinical

Follow-up

more than 1.5 years

Authors

XXXiao XiaoGuangdong Academy of Medical Sciences JLJing LiGuangzhou University of Chinese Medicine RSR. Jude SamulskiUniversity of North Carolina at Chapel Hill

Discussion

Member takes

Implication

Supports durable rAAV muscle transduction in mice; leaves open human translation and clinical trials.

Key Points

To evaluate the efficiency, longevity, and immunogenicity of recombinant adeno-associated virus (rAAV) vectors for in vivo gene transfer into mammalian muscle tissue.
Introduced a recombinant adeno-associated virus vector carrying a lacZ reporter gene into the muscle tissue of immunocompetent mice.
Evaluated reporter expression, host cellular immune responses, and molecular vector persistence for over 1.5 years.
Achieved efficient lacZ transduction and robust transgene expression persisting for more than 1.5 years without inducing a cellular immune response.
Identified vector episome conversion into high-molecular-weight genomic DNA as the underlying mechanism for long-term persistence.

Structured PICO

Does recombinant adeno-associated virus vector (rAAV) achieve long-term gene transduction in muscle tissue of immunocompetent mice?

Population

Immunocompetent mice

Intervention

Recombinant adeno-associated virus vector (rAAV) carrying a lacZ reporter introduced into muscle tissue

Outcome

Gene transduction, expression persistence, and cellular immune responsesurrogate

Recombinant adeno-associated virus vectors can establish efficient, long-term gene transduction in mammalian muscle cells in vivo without requiring immune modulation.

Cite This Study

Xiao et al. (1996) studied this question.

synapsesocial.com/papers/69ffbcaab124fe5819859709 https://doi.org/10.1128/jvi.70.11.8098-8108.1996

View Full Paper

Ask AI

Bookmark

Efficient long-term gene transfer into muscle tissue of immunocompetent mice by adeno-associated virus vector

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Efficient long-term gene transfer into muscle tissue of immunocompetent mice by adeno-associated virus vector

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider