December 1, 1995Journal of Biological ChemistryOpen Access

Chimeric Substitutions of the Actin-binding Loop Activate Dephosphorylated but Not Phosphorylated Smooth Muscle Heavy Meromyosin

View Full Paper

Ask AI

Bookmark

Population

Smooth muscle heavy meromyosin (HMM)

Comparison

Substitution of the native actin-binding loop… vs Wild-type smooth muscle HMM

Design

Preclinical

Authors

ARArthur S. RovnerUniversity of Vermont YFYelena FreyzonBroad Institute KTKathleen M. TrybusUniversity of Vermont

Discussion

Member takes

Implication

Highlights structural control of myosin activation; hypothesis-generating for contractility research, no clinical translation yet.

Structured PICO

Population

Smooth muscle heavy meromyosin (HMM)

Intervention

Substitution of the native actin-binding loop with that from skeletal or beta-cardiac myosin

Comparator

Wild-type smooth muscle HMM

Outcome

Actin-activated ATPase activity and actin motilitysurrogate

Substitution of the actin-binding loop in smooth muscle myosin with striated muscle sequences bypasses the need for regulatory light chain phosphorylation for activation.

Cite This Study

Rovner et al. (1995) studied this question.

synapsesocial.com/papers/69ffccafd1d8b50f8e9a12f2 https://doi.org/10.1074/jbc.270.51.30260

View Full Paper

Ask AI

Bookmark

Chimeric Substitutions of the Actin-binding Loop Activate Dephosphorylated but Not Phosphorylated Smooth Muscle Heavy Meromyosin

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Chimeric Substitutions of the Actin-binding Loop Activate Dephosphorylated but Not Phosphorylated Smooth Muscle Heavy Meromyosin

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider