August 1, 1994Journal of Biological ChemistryOpen Access

Regulation of expressed truncated smooth muscle myosins. Role of the essential light chain and tail length.

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Population

Truncated smooth muscle myosin expressed via a baculovirus expression system

Comparison

Substitution of essential light chain with… vs Control heavy meromyosin containing native RLC…

Design

Preclinical

Authors

KTKathleen M. TrybusUniversity of Vermont

Discussion

Member takes

Implication

No immediate clinical implications; leaves open whether tail-dependent myosin regulation translates to human vascular smooth muscle.

Structured PICO

Population

Truncated smooth muscle myosin expressed via a baculovirus expression system

Intervention

Substitution of essential light chain (ELC) with smooth ELC, skeletal ELC, or no ELC; substitution of skeletal RLC for smooth RLC; truncation of tail from 72 to 27 nm

Comparator

Control heavy meromyosin (HMM) containing native RLC and ELC

Outcome

Movement of actin in a motility assay (rate and phosphorylation dependence)surrogate

Molecular changes induced at the active site of smooth muscle myosin by phosphorylation can occur independent of the ELC but critically depend on a stable coiled-coil tail.

Cite This Study

Kathleen M. Trybus (1994) studied this question.

synapsesocial.com/papers/6a7372e820644e164b4e5b89 https://doi.org/10.1016/s0021-9258(17)31894-x

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