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May 10, 2026Nucleic Acids Research0 citationsOpen Access

Tetracycline-Inducible Framework for Regulating Gene Expression in Mammals

Development of a tetracycline-inducible programmed ribosomal frameshifting platform for sensitive regulation of mammalian gene expression

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Authors

SKSıla KöseEMElisabeth MüllerPGPierre Gommeringer

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Overview

Randomized trial demonstrates precise gene expression control in mammals, indicating promise for advanced therapies.

Key Points

  • This research aims to develop a tetracycline-inducible riboswitch platform for sensitive regulation of gene expression in mammalian systems.
  • Developed a tetracycline-inducible riboswitch utilizing a modified -1 programmed ribosomal frameshifting platform.
  • Conducted dose-response analysis revealing an EC50 of 0.41 μM for sensitivity.
  • Validated the platform's effectiveness across various genetic contexts to confirm modularity.
  • Achieved robust induction of gene expression through ligand addition with optimized riboswitches.
  • Reversed gene expression observed, demonstrating the platform's beneficial characteristics.
  • The -1 PRF platform exhibited exceptional sensitivity compared to existing systems.

Cite This Study

Köse et al. (2026) studied this question.

synapsesocial.com/papers/6a0021e6c8f74e3340f9ce4bhttps://doi.org/10.1093/nar/gkag426
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