What question did this study set out to answer?

Identify substrate-binding regions across cytochrome P450 family 2 (CYP2) enzymes and evaluate their patterns of evolutionary variation.

January 1, 1992Journal of Biological ChemistryOpen Access

Substrate recognition sites in cytochrome P450 family 2 (CYP2) proteins inferred from comparative analyses of amino acid and coding nucleotide sequences.

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Authors

OGOsamu GotohJapanese Foundation For Cancer Research

Discussion

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Implication

Comparative sequence analysis reveals six substrate recognition sites in cytochrome P450 family 2 proteins, indicating accelerated genetic diversification to handle novel foreign compounds.

Key Points

Identify substrate-binding regions across cytochrome P450 family 2 (CYP2) enzymes and evaluate their patterns of evolutionary variation.
Performed group-to-group sequence alignments between CYP2 family members and bacterial P450 enzymes with crystallographically defined substrate-binding sites.
Mapped published point mutations and chimeric segments affecting CYP2 substrate specificity onto the aligned sequences.
Evaluated nucleotide substitution dynamics and nonsynonymous substitution rates across CYP2A, CYP2B, CYP2C, and CYP2D subfamilies.
Identified six putative substrate recognition sites (SRSs) dispersed along the primary sequence, constituting approximately 16% of total amino acid residues.
All previously reported point mutations and chimeric fragments altering substrate specificity mapped directly to or overlapped these six SRS regions.
The six SRSs exhibited significantly higher rates of nonsynonymous nucleotide substitutions than the remainder of the coding sequences across examined subfamilies.

Cite This Study

Osamu Gotoh (1992) studied this question.

synapsesocial.com/papers/6a006eecda5c1eb07f2daa23 https://doi.org/10.1016/s0021-9258(18)48462-1

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Implication

Comparative sequence analysis reveals six substrate recognition sites in cytochrome P450 family 2 proteins, indicating accelerated genetic diversification to handle novel foreign compounds.

Substrate recognition sites in cytochrome P450 family 2 (CYP2) proteins inferred from comparative analyses of amino acid and coding nucleotide sequences.

Discussion

Member takes

Implication

Key Points

Cite This Study

Substrate recognition sites in cytochrome P450 family 2 (CYP2) proteins inferred from comparative analyses of amino acid and coding nucleotide sequences.

Discussion

Member takes

Implication

Key Points

Cite This Study

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