What question did this study set out to answer?

To determine the molecular mechanism by which ganglioside GM1 enhances the biological actions of nerve growth factor (NGF) through the Trk receptor.

May 23, 1995Proceedings of the National Academy of SciencesOpen Access

Ganglioside GM1 binds to the Trk protein and regulates receptor function.

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Authors

TMTatsuro MutohFujita Health University ATAkira TokudaKanazawa University TMToshiaki MiyadaiFukui Prefectural University

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Implication

In vitro study reveals ganglioside GM1 binds to Trk and enhances nerve growth factor signaling in rat PC12 cells, indicating GM1 functions as an endogenous receptor activator.

Key Points

To determine the molecular mechanism by which ganglioside GM1 enhances the biological actions of nerve growth factor (NGF) through the Trk receptor.
Assessed neurite outgrowth, neurofilament expression, and receptor autophosphorylation in rat PC12 cells treated with GM1 and low-dose NGF.
Conducted in vitro receptor binding and autophosphorylation assays using immunoprecipitated Trk, p75NGFR, and epidermal growth factor receptor (EGFR) exposed to monosialoganglioside GM1 or polysialogangliosides.
GM1 tightly and specifically associated with Trk (but not p75NGFR or EGFR), producing a >3-fold increase in NGF-induced Trk autophosphorylation compared with NGF alone.
GM1 strongly stimulated neurite outgrowth and neurofilament expression in PC12 cells at low NGF concentrations that were otherwise insufficient to trigger neuronal differentiation.

Cite This Study

Mutoh et al. (1995) studied this question.

synapsesocial.com/papers/6a00d472831589f3542deebb https://doi.org/10.1073/pnas.92.11.5087

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Implication

In vitro study reveals ganglioside GM1 binds to Trk and enhances nerve growth factor signaling in rat PC12 cells, indicating GM1 functions as an endogenous receptor activator.

Ganglioside GM1 binds to the Trk protein and regulates receptor function.

Discussion

Member takes

Implication

Key Points

Cite This Study

Ganglioside GM1 binds to the Trk protein and regulates receptor function.

Discussion

Member takes

Implication

Key Points

Cite This Study

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