What question did this study set out to answer?

To determine the presence, structural distribution, and clinical relevance of neutrophils, neutrophil extracellular traps (NETs), and interleukins 17A and 17F across stages of coronary thrombus organization following acute myocardial infarction.

December 14, 2012Thrombosis and Haemostasis

Neutrophils, neutrophil extracellular traps and interleukin-17 associate with the organisation of thrombi in acute myocardial infarction

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Authors

XLXiaofei LiThomas Jefferson University PTPeter TeelingAmsterdam UMC Location University of Amsterdam CMClaire MackaayAmsterdam UMC Location University of Amsterdam

Discussion

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Implication

Histopathological analysis reveals abundant neutrophil extracellular traps and interleukin-17 in fresh coronary thrombi from myocardial infarction patients, suggesting a role in clot stabilization.

Key Points

To determine the presence, structural distribution, and clinical relevance of neutrophils, neutrophil extracellular traps (NETs), and interleukins 17A and 17F across stages of coronary thrombus organization following acute myocardial infarction.
Examined 45 coronary thrombosuction specimens retrieved from patients after acute myocardial infarction, categorized into 15 fresh, 15 lytic, and 15 organised thrombi.
Identified neutrophils and NETs using hematoxylin-eosin, Feulgen staining, and immunohistochemistry for histone H1, myeloperoxidase (MPO), and neutrophil elastase.
Evaluated IL-17A and IL-17F localization and protein expression using double (immuno)histochemical staining with spectral image analysis, rtPCR, and Western blotting.
Neutrophils constituted 10% to 30% of thrombus mass in fresh and lytic thrombi but were absent in organized thrombi.
NETs were identified in 4 of 15 fresh thrombi and 12 of 15 lytic thrombi, but were completely absent (0 of 15) in organized thrombus specimens.
Cytoplasmic IL-17A and IL-17F colocalized with DNA within the majority of neutrophils, extracellular areas, and NETs, with protein expression confirmed by Western blot.

Cite This Study

Li et al. (2012) studied this question.

synapsesocial.com/papers/6a01719a0ed7d2e5335c94bb https://doi.org/10.1160/th12-06-0425

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Implication

Histopathological analysis reveals abundant neutrophil extracellular traps and interleukin-17 in fresh coronary thrombi from myocardial infarction patients, suggesting a role in clot stabilization.

Neutrophils, neutrophil extracellular traps and interleukin-17 associate with the organisation of thrombi in acute myocardial infarction

Discussion

Member takes

Implication

Key Points

Cite This Study

Neutrophils, neutrophil extracellular traps and interleukin-17 associate with the organisation of thrombi in acute myocardial infarction

Discussion

Member takes

Implication

Key Points

Cite This Study

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