DNA methylation mediates MBSR induced cardioprotection in patients after PCI

Figures

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  Fig. 1. Study design and molecular analysis workflow. (a) Flowchart of the matched-control study design. (b) Genome-wide DNA methylation analysis workflow.

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  Fig. 2. Reliability of DNA Methylation Profiling across Groups. (a) Pearson correlation coefficients of methylation read counts between baseline, 6 months post-MBSR, and control samples; (b) Spearman correlation coefficients of methylation patterns across groups; (c) Heatmap showing hierarchical clustering of methylation profiles, highlighting intra-group consistency and inter-group separation.

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  Fig. 3. KEGG Pathway Enrichment Analysis. (a) Intra-group (post-MBSR vs. pre-MBSR) KEGG Pathway Enrichment Bar Plot; (b) Inter-group (post-MBSR vs. control) KEGG Pathway Enrichment Bar Plot. Pathways shown are those with nominal enrichment (raw P < 0.1) before multiple comparison correction. After Benjamini‑Hochberg FDR correction, no pathway remained statistically significant (all padj > 0.05). KEGG pathway maps were generated with permission from Kanehisa Laboratories25.

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  Fig. 4. Protein-Protein Interaction (PPI) Networks of Differentially Methylated Genes. (a) PPI Network of Immune-Inflammation Module (Intra-group: post-MBSR vs. pre-MBSR). (b) PPI Network of Coagulation-Metabolism Module (Inter-group: post-MBSR vs. control).

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  Fig. 5. Venn Diagram Analysis of Methylome-Transcriptome Overlap. (a) Inter-group Overlap: Hypomethylated Genes and Upregulated RNAs (post-MBSR vs. Control. (b) Intra-group Overlap: Hypomethylated Genes and Upregulated RNAs (pre- vs. post-MBSR). (c) Inter-group Overlap: Hypermethylated Genes and Downregulated RNAs (post-MBSR vs. Control). (d) Intra-group Overlap: Hypermethylated Genes and Downregulated RNAs (pre- vs. post-MBSR).