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Elucidate the complete biosynthetic pathway of the plant antioxidant carnosic acid and functionally reconstitute it inside yeast cells.

October 5, 2016Nature CommunicationsOpen Access

Elucidation of the biosynthesis of carnosic acid and its reconstitution in yeast

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Authors

USUlschan Scheler WBWolfgang BrandtLeibniz Institute of Plant Biochemistry APAndrea PorzelHanoi National University of Education

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Implication

Biochemical study demonstrates complete reconstitution of carnosic acid biosynthesis in yeast, highlighting opportunities for microbial production of therapeutic phenolic diterpenes.

Key Points

Elucidate the complete biosynthetic pathway of the plant antioxidant carnosic acid and functionally reconstitute it inside yeast cells.
Characterized cytochrome P450 oxygenases (CYP76AH and CYP76AK families) isolated from Rosmarinus officinalis, Salvia fruticosa, and Salvia miltiorrhiza.
Used structure-based protein modeling and mutagenesis of three residues to convert CYP76AH1 into an active 11-hydroxyferruginol synthase.
Reconstituted the complete multienzyme oxidative pathway in yeast to achieve de novo carnosic acid biosynthesis.
Identified that CYP76AH22-24 enzymes from rosemary and sage catalyze the oxidation of ferruginol to 11-hydroxyferruginol.
Demonstrated that CYP76AK6-8 enzymes catalyze three consecutive C20 oxidation steps converting 11-hydroxyferruginol into carnosic acid, enabling successful pathway engineering in yeast.

Cite This Study

Scheler et al. (2016) studied this question.

synapsesocial.com/papers/6a019b61897643a80dcaf6a5 https://doi.org/10.1038/ncomms12942

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Implication

Biochemical study demonstrates complete reconstitution of carnosic acid biosynthesis in yeast, highlighting opportunities for microbial production of therapeutic phenolic diterpenes.

Elucidation of the biosynthesis of carnosic acid and its reconstitution in yeast

Discussion

Member takes

Implication

Key Points

Cite This Study

Elucidation of the biosynthesis of carnosic acid and its reconstitution in yeast

Discussion

Member takes

Implication

Key Points

Cite This Study

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