Camystat: a high-throughput open-source software tool analysis of contractile cardiomyocytes recordings

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  Figure 1. (A) Interface of the Camystat software running on Windows. Shown are the available analytical modules and export options, including raw output (CSV), interactive plots, heatmaps, and summary statistical reports. (B) Schematic representation of a single contraction–relaxation cycle: (I) area under the curve corresponding to the contraction phase; (ii) area under the curve corresponding to the relaxation phase; (iii) baseline noise floor between contractile events. (C) Differential motion-analysis workflow. Consecutive frames (n and n + 1) are first binarized (i), after which inter-frame displacement is quantified using an XOR operator (ii). The final panel presents a representative motion-derived signal generated by Camystat, illustrating temporal changes in pixel-level differences (iii). (D) Spearman rank-correlation heatmap comparing image-analysis algorithms. Correlations were computed using 61 388 paired measurements derived from all frames across 25 independent cardiomyocyte recordings (frame-level observations treated as technical replicates nested within recordings). (E) Comparison of computational performance across algorithms. Each distribution represents execution times…