What question did this study set out to answer?

To determine whether reactive oxygen species generation during complex I substrate oxidation originates primarily from complex I or complex III in intact cardiac mitochondria.

September 1, 2003Journal of Biological ChemistryOpen Access

Production of Reactive Oxygen Species by Mitochondria

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Authors

QCQun ChenKunming University of Science and Technology EVEdwin J. VazquezCentral Michigan University SMShadi MoghaddasUniversity of Houston

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Implication

Preclinical study demonstrates complex III drives reactive oxygen species production in rat cardiac mitochondria, indicating rotenone protects cells by limiting downstream electron transfer.

Key Points

To determine whether reactive oxygen species generation during complex I substrate oxidation originates primarily from complex I or complex III in intact cardiac mitochondria.
Isolated intact mitochondria and submitochondrial particles from Sprague-Dawley rat hearts.
Measured reactive oxygen species generation as hydrogen peroxide using the amplex red fluorometric assay in the presence of respiratory substrates, rotenone, antimycin A, and catalase.
In intact mitochondria oxidizing complex I substrates, rotenone did not increase hydrogen peroxide, whereas antimycin A markedly increased production unless co-treated with rotenone.
In submitochondrial particles lacking matrix antioxidant defenses, complex I inhibition with rotenone markedly increased hydrogen peroxide generation during NADH oxidation.
Complex III acts as the primary site of detectable reactive oxygen species release in intact mitochondria, while complex I-derived reactive oxygen species are scavenged by internal matrix defenses.

Cite This Study

Chen et al. (2003) studied this question.

synapsesocial.com/papers/6a02a2cf67f6ea5cc8755068 https://doi.org/10.1074/jbc.m304854200

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Implication

Preclinical study demonstrates complex III drives reactive oxygen species production in rat cardiac mitochondria, indicating rotenone protects cells by limiting downstream electron transfer.

Production of Reactive Oxygen Species by Mitochondria

Discussion

Member takes

Implication

Key Points

Cite This Study

Production of Reactive Oxygen Species by Mitochondria

Discussion

Member takes

Implication

Key Points

Cite This Study

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