October 30, 1992Science
Comparative Genomic Hybridization for Molecular Cytogenetic Analysis of Solid Tumors
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Implication
Molecular cytogenetic analysis uncovers novel DNA sequence copy number amplifications in bladder tumors, demonstrating a genome-wide method to map chromosomal alterations.
Key Points
- To develop comparative genomic hybridization as a genome-wide method to map DNA sequence copy number variations across chromosomes in solid tumors.
- Simultaneously hybridized differentially labeled tumor test DNA and normal reference DNA onto normal metaphase chromosome spreads.
- Quantified ratio changes in fluorescence intensities between two distinct fluorochromes along target chromosomes to detect copy number gains, losses, and amplifications.
- Applied the assay to tumor cell lines and primary bladder tumor specimens.
- Identified 16 distinct chromosomal regions of DNA amplification across tumor cell lines and primary bladder tumors.
- Detected multiple amplified gene loci that were not previously recognized as amplified in these tumor types.
Cite This Study
Kallioniemi et al. (1992) studied this question.