Polyacrylamide-based, ratiometric pH nanosensor particles with a mean diameter of 50 nm were prepared by microemulsion polymerization. A pH sensitive dye, a fluoresceinamine derivative, and a pH insensitive reference dye, a rhodamine B derivative, were covalently linked to the nanoparticle matrix by copolymerization to provide a stable and robust nanosensor. The dyes are distributed throughout the matrix.
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Sun et al. (2006) studied this question.
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