Atg11 Links Cargo to the Vesicle-forming Machinery in the Cytoplasm to Vacuole Targeting Pathway
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Implication
Molecular study reveals Atg11 coordinates cargo delivery to the pre-autophagosomal structure in yeast, suggesting cargo actively directs vesicle-forming machinery assembly.
Key Points
- To identify the structural domains of Atg11 that mediate cargo binding and transport to the site of vesicle formation in the cytoplasm-to-vacuole targeting pathway.
- Conducted domain mapping of Atg11 to evaluate its C-terminus, central regions, and coiled-coil domains.
- Assayed protein-protein interactions and self-assembly among Atg11, Atg17, Atg20, and the Atg19–precursor Ape1 cargo complex.
- Characterized Atg11 mutant strains to track transport of the cargo complex and the subcellular localization of core autophagy components.
- Mapped the binding of the cargo receptor complex specifically to the Atg11 C-terminus, while central regions mediated pre-autophagosomal structure recruitment.
- Identified that Atg11 coiled-coil domains direct self-assembly and bind novel interaction partners Atg17 and Atg20.
- Demonstrated that transport-defective Atg11 mutants alter the localization of other Atg proteins, confirming that cargo recruitment helps organize vesicle-forming machinery.
Cite This Study
Yorimitsu et al. (2005) studied this question.