What question did this study set out to answer?

To determine how TLR7 and TLR8 signaling regulates intestinal dendritic cell migration and functional activation in vivo.

May 1, 2006The Journal of ImmunologyOpen Access

Regulation of Intestinal Dendritic Cell Migration and Activation by Plasmacytoid Dendritic Cells, TNF-α and Type 1 IFNs after Feeding a TLR7/8 Ligand

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Authors

UYUlf YrlidUniversity of Gothenburg SMSimon MillingUniversity of Glasgow JMJoanna L. MillerUniversity of Oxford

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Implication

Animal study reveals oral TLR7/8 activation drives intestinal dendritic cell migration and maturation via plasmacytoid dendritic cells, indicating indirect cytokine control of mucosal immunity.

Key Points

To determine how TLR7 and TLR8 signaling regulates intestinal dendritic cell migration and functional activation in vivo.
Administered the oral TLR7/8 ligand resiquimod (R-848) to rats and mice.
Sampled and analyzed dendritic cells in pseudoafferent intestinal lymph (rats) and mesenteric lymph nodes (rats and mice).
Assessed surface marker expression (CD86, CD25), cytokine dependence (TNF-alpha and type 1 IFNs), and the consequences of plasmacytoid dendritic cell depletion.
Oral R-848 induced a 20- to 30-fold increase in intestinal dendritic cell output within 10 hours, resulting in a completely TNF-alpha-dependent accumulation of dendritic cells in mouse mesenteric lymph nodes.
Migrating intestinal lymph dendritic cells selectively up-regulated CD25 without CD86, whereas mesenteric lymph node dendritic cells up-regulated CD86 through a type 1 IFN-dependent mechanism.
Depleting plasmacytoid dendritic cells significantly reduced the systemic cytokine surge, lymph node dendritic cell accumulation, and CD86 activation.

Cite This Study

Yrlid et al. (2006) studied this question.

synapsesocial.com/papers/6a036e550b0c10f6aaaee04f https://doi.org/10.4049/jimmunol.176.9.5205

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Implication

Animal study reveals oral TLR7/8 activation drives intestinal dendritic cell migration and maturation via plasmacytoid dendritic cells, indicating indirect cytokine control of mucosal immunity.

Regulation of Intestinal Dendritic Cell Migration and Activation by Plasmacytoid Dendritic Cells, TNF-α and Type 1 IFNs after Feeding a TLR7/8 Ligand

Discussion

Member takes

Implication

Key Points

Cite This Study

Regulation of Intestinal Dendritic Cell Migration and Activation by Plasmacytoid Dendritic Cells, TNF-α and Type 1 IFNs after Feeding a TLR7/8 Ligand

Discussion

Member takes

Implication

Key Points

Cite This Study

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