March 1, 1993Journal of Histochemistry & Cytochemistry

Conventional and confocal fluorescence microscopy of collagen fibers in the heart.

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Population

Heart tissue (collagen fibers in perimysial and endomysial septa)

Comparison

Phosphomolybdic acid treatment before… vs Brightfield PSR or PMA-PSR method

Design

Preclinical

Authors

PDPaul C. DolberPennsylvania State University MSMadison S. SpachPediatric / Congenital Cardiology

Discussion

Member takes

Implication

Enhances preclinical 3D collagen imaging in animal hearts; leaves open translation to human cardiac histopathology.

Structured PICO

Population

Heart tissue (collagen fibers in perimysial and endomysial septa)

Intervention

Phosphomolybdic acid (PMA) treatment before picrosirius red (PSR) staining visualized by fluorescence and confocal microscopy

Comparator

Brightfield PSR or PMA-PSR method

Outcome

Visualization and sensitivity of collagen fiber three-dimensional arrangementsurrogate

The PMA-PSR fluorescence method significantly improves the visualization of 3D collagen fiber arrangements in the heart compared to traditional brightfield microscopy.

Cite This Study

Dolber et al. (1993) studied this question.

synapsesocial.com/papers/6a03aede94ec7ec37ca9c782 https://doi.org/10.1177/41.3.7679127

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