Profiling of Single Cells on a Droplet Microfluidics–SERS Platform for Multiplexed Analysis of Oxidative and Inflammatory Stress

was monitored through the responsive spectral changes of 4-MPBA on the reporter probe, whereas IL-6 detection relied on target-mediated sandwich immunocomplex formation between the reporter and capture probes, resulting in SERS signal amplification. Single-cell measurements reveal pronounced oxidative and inflammatory activation in MDA-MB-231 cells, whereas SK-BR-3 cells display attenuated responses, highlighting subtype-specific heterogeneity. In addition, cisplatin treatment further modulated the stress-response patterns induced by electrical stimulation, indicating distinct subtype-dependent adaptability to combined physical and chemical perturbations. This platform demonstrates potential for broader applications in single-cell analysis, providing a powerful tool for dissecting cell-specific stress and inflammatory dynamics.