What does this research mean for the field?

A combined electrochemical-LSPR multiplexed immunoassay utilizing distinct redox probes and a dimensionless multi-analyte index enables the simultaneous, sensitive, and specific quantification of the sepsis-associated biomarkers PCT, CRP, and SAA in human serum. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

To develop and validate an electrochemical and localized surface plasmon resonance multiplexed immunoassay coupled with a multi-analyte index for detecting sepsis-related biomarkers.

September 8, 2026ACS Measurement Science AuOpen Access

Electrochemical–LSPR Multiplexed Immunoassay with a Multi-Analyte Index for Sepsis-Associated Biomarkers

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Authors

ZBZoe Bradley AGAbhijit Ganguly PPPagona Papakonstantinou

Discussion

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Overview

Analytical validation study demonstrates multiplexed detection of sepsis biomarkers in human serum, highlighting a standardized multianalyte index for rapid infection profiling.

Key Points

To develop and validate an electrochemical and localized surface plasmon resonance multiplexed immunoassay coupled with a multi-analyte index for detecting sepsis-related biomarkers.
Configured a four-working-electrode screen-printed carbon electrode functionalized with gold nanoparticles and individual redox probes (methylene blue, cysteine, and ruthenium hexamine chloride) for differential pulse voltammetry.
Integrated localized surface plasmon resonance (LSPR) optical measurements as an orthogonal modality to confirm antibody–antigen binding.
Analyzed calibration ranges, analytical sensitivity, and cross-reactivity for procalcitonin (PCT), C-reactive protein (CRP), and serum amyloid A (SAA) across PBS and 50% human serum across replicate runs (n ≥ 3).
Achieved limits of detection in PBS of 0.0255 ng mL–1 for PCT, 1.60 μg mL–1 for CRP, and 0.0670 μg mL–1 for SAA across calibration ranges of 0.5–20 ng mL–1 (PCT) and 0.5–20 μg mL–1 (CRP, SAA).
Demonstrated limits of detection in 50% human serum of 6.99 ng mL–1 for PCT, 0.0556 μg mL–1 for CRP, and 0.193 μg mL–1 for SAA with minimal cross-reactivity.
Devised a dimensionless multianalyte index (MAI) based on normalized biomarker concentrations to synthesize heterogeneous sensor signals into an integrated analytical metric.

Cite This Study

Bradley et al. (2026) studied this question.

synapsesocial.com/papers/6a9fd75858e84d0ff5b45f16 https://doi.org/10.1021/acsmeasuresciau.6c00255

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September 8, 2026ACS Measurement Science AuOpen Access

Electrochemical–LSPR Multiplexed Immunoassay with a Multi-Analyte Index for Sepsis-Associated Biomarkers

View Full Paper

Ask AI

Bookmark

Authors

ZBZoe Bradley AGAbhijit Ganguly PPPagona Papakonstantinou

Discussion

Member takes

Overview

Analytical validation study demonstrates multiplexed detection of sepsis biomarkers in human serum, highlighting a standardized multianalyte index for rapid infection profiling.

Key Points

To develop and validate an electrochemical and localized surface plasmon resonance multiplexed immunoassay coupled with a multi-analyte index for detecting sepsis-related biomarkers.
Configured a four-working-electrode screen-printed carbon electrode functionalized with gold nanoparticles and individual redox probes (methylene blue, cysteine, and ruthenium hexamine chloride) for differential pulse voltammetry.
Integrated localized surface plasmon resonance (LSPR) optical measurements as an orthogonal modality to confirm antibody–antigen binding.
Analyzed calibration ranges, analytical sensitivity, and cross-reactivity for procalcitonin (PCT), C-reactive protein (CRP), and serum amyloid A (SAA) across PBS and 50% human serum across replicate runs (n ≥ 3).
Achieved limits of detection in PBS of 0.0255 ng mL–1 for PCT, 1.60 μg mL–1 for CRP, and 0.0670 μg mL–1 for SAA across calibration ranges of 0.5–20 ng mL–1 (PCT) and 0.5–20 μg mL–1 (CRP, SAA).
Demonstrated limits of detection in 50% human serum of 6.99 ng mL–1 for PCT, 0.0556 μg mL–1 for CRP, and 0.193 μg mL–1 for SAA with minimal cross-reactivity.
Devised a dimensionless multianalyte index (MAI) based on normalized biomarker concentrations to synthesize heterogeneous sensor signals into an integrated analytical metric.

Cite This Study

Bradley et al. (2026) studied this question.

synapsesocial.com/papers/6a9fd75858e84d0ff5b45f16 https://doi.org/10.1021/acsmeasuresciau.6c00255

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