In vitro study shows nanoparticle delivery of DNAzyme Dz13 inhibits proliferation and migration in HepG2 liver cancer cells, suggesting a viable platform for targeted gene therapy.
BACKGROUND: The phenylboronic acid-functionalized polyamidoamine (PP) was employed as a gene carrier for Dz13 delivery, inducing an obvious anticancer response. MATERIALS AND METHODS: The Dz13 condensation ability of PP was evaluated through gel retardation assay. The cellular uptake mechanism of PP/Dz13 nanoparticles was studied using confocal laser scanning microscope and flow cytometer. The inhibition ability of cell proliferation, migration and invasion was investigated through MTT assay, flow cytometry, wound healing and Transwell migration assays, using hepatocarcinoma cell line HepG2 as a model. Finally, Western blotting analysis was used to detect the signaling pathway associated with the inhibition of cell apoptosis and migration induced by Dz13 delivery. CONCLUSION: The PP provided a potential delivery system to achieve the tumor-targeting gene therapy.
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Yang et al. (2019) studied this question.
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