Whereas morphological analysis of populations recognized as Leptodactylus mystaceus indicates there is one species with modest geographic variation, analysis of advertisement calls indicates there are at least two or perhaps three species involved. The differences found in advertisement calls are sufficient to act as species isolating barriers to recognize at least two species, which action is taken. A consequent result is the description of a new sibling species. The significance of sibling species in the genus Leptodacytylus is discussed briefly.
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Heyer et al. (1996) studied this question.
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