Key result
Yeast-expressed recombinant bovine interferon alpha 1 exhibits typical antiviral activity and high stability.
Why the study?
Does recombinant bovine interferon α1 exhibit antiviral activity and typical physicochemical characteristics of IFNα in vitro?
Does recombinant bovine interferon α1 exhibit antiviral activity and typical physicochemical characteristics of IFNα in vitro?
Answers are grounded in this paper and cite their sources.
Recombinant bovine interferon α1 expressed in yeast exhibits typical physicochemical characteristics and significant in vitro antiviral activity against vesicular stomatitis virus.
A bovine interferon α (BoIFNα) gene that included signal sequence was amplified from bovine liver genomic DNA. The gene was named BoIFN-α1 according to the position at which the encoded gene of the bovine IFN was located in the bovine genome. The sequence included a 23-amino-acid signal peptide and a 166-amino-acid mature peptide. The structural characteristics and phylogenetic relationships of the BoIFN-α1 gene were analyzed. A recombinant mature BoIFN-α1 (rBoIFN-α1) was expressed in the yeast Pichia pastoris. Physicochemical characteristics and antiviral activity were determined in vitro. Recombinant BoIFN-α1 was found to be highly sensitive to trypsin and stable at pH 2.0 or 65°C. It also exhibited antiviral activity, which was neutralized by a rabbit anti-rBoIFNα polyclonal antibody. This study revealed that rBoIFN-α1 has the typical characteristics of IFNα and can be used for both research and industrial application.
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Shao et al. (2014) studied this question. Recombinant mature BoIFN-α1 was evaluated on Physicochemical characteristics and antiviral activity. Recombinant bovine interferon alpha 1 expressed in yeast exhibited typical IFN-alpha characteristics, including antiviral activity, high sensitivity to trypsin, and stability at pH 2.0 or 65°C.
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