Answers are grounded in this paper and cite their sources.
High resolution purification procedures for the separation and characterization of neuropeptides are described. The methods, which are complementary, are based on ion exchange chromatography and reverse phase high pressure liquid chromatography and are designed to be used in conjunction with radioimmunoassays and bioassays for known peptides or structural identification in the case of peptides of unknown sequence. The high pressure liquid chromatographic method is versatile, highly reproducible and applicable to picomolar quantities of peptides and it gives very high resolution between most of the neuropeptides of present‐day interest. The applicability of the method to the study of crude brain extracts is demonstrated.
No takes yet. Share an insight, caveat, or question.
Morris et al. (1980) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: