Chemiluminescent Probe Enables Enhanced Visualization of Acute Ischemic Stroke via Acetylcholinesterase Activation

imaging with AhCL produced a 3-23-fold enhancement in high-contrast chemiluminescent signals across differentially hypoxia-exposed brain tissues within 1 h, clearly distinguishing tissue hypoxia levels. Notably, AChE activation allowed the probe to effectively differentiate hypoxia-induced brain injury, potentially providing critical guidance for clinical intervention. This study establishes AChE as a robust biomarker for AIS and offers a potential tool for timely diagnosis and assessment.