Performance of Wild Saccharomyces cerevisiae Strains in Enriched-Dough Kouglof

Two wild Saccharomyces cerevisiae strains, 9-3 and 10-2, isolated from nectarine and apple leaves, show high fermentation performance in standard breadmaking but their utility in enriched-dough products remained untested. This study evaluated their performance in kouglof production against a commercial baker’s yeast as a control. Fermentation was monitored by weight loss (reflecting CO2 production) and pH changes. Finished loaves were evaluated for macroscopic traits (weight, height, appearance), crust/crumb color, texture, and microstructure via 3D/2D analysis of gas-cell morphology. Sensory acceptance was also assessed. While size-related traits were similar across all samples, strain 10-2 significantly outperformed 9-3. Kouglof made with 10-2 exhibited commercial-grade softness, characterized by lower hardness and gumminess, lower residual sugar content, and a finer, more uniform gas-cell structure with a darker, well-developed crust, whereas 9-3 yielded a firmer crumb with fewer, larger gas cells. Sensory acceptance was comparable across variants, with all samples rated as highly acceptable. Strain 10-2 demonstrated high sucrose tolerance and performed comparably to or better than the control across multiple product-quality metrics in enriched-dough kouglof. These findings support the potential of wild yeast 10-2 for sweet bread production with high-sucrose and high-fat formulations, though further optimization is warranted.