Synergistic Probe Combining Lysosome Anchoring and Tumor Defense System Targeting for Precise Tumor Visualization

by introducing a γ-glutamyltransferase substrate, following our group's prior offensive and defensive integration strategy. Due to its smart design, CN-D-GGT, upon activation, shows marked changes in fluorescence and photoacoustic signals, enabling its application for multi-modal imaging. It also has high specificity, distinguishing cancer cells in co-culture (∼6-fold) and clearly differentiating tumors from normal and inflamed tissues (T/N or T/I signal ratios > 3.5). Importantly, it can also differentiate cancerous from adjacent tissues in clinical samples. This work has developed a probe that can accurately light tumors in complex pathological environments, with the potential to assist in intraoperative resection decision-making, avoid excessive or missed resection.