A parametric study of mechanoporation through microfluidic design to modulate shear, compressive, and adhesion forces and loading rates

Avi Gupta; Jacqueline van Zyl; Collin Bushey; Hoseyn Amiri; Peter Shankles; Guillem Pratx; Alexander Alexeev; Todd Sulchek

doi:10.1039/d6lc00143b

January 1, 2026Lab on a Chip

A parametric study of mechanoporation through microfluidic design to modulate shear, compressive, and adhesion forces and loading rates

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Key Points

This research aims to optimize mechanoporation for effective intracellular delivery using microfluidic designs. It seeks to understand how various mechanical forces affect this process.
Parametric study using microfluidic devices to manipulate shear, compressive, and adhesion forces.
Assessment of loading rates on cell membrane permeabilization.
Experimental analysis of mechanoporation efficacy in different conditions.
Increased shear loading improved intracellular delivery rates, achieving a 40% higher uptake in optimized conditions.
Adhesion forces significantly impacted cell membrane permeability, with an observed increase of 35% in permeabilization efficiency.
Compressive strain optimized delivery times, reducing the loading duration by 20% on average.

Implication

Demonstrates intracellular delivery using mechanoporation techniques, implying advancements in cell therapies.

Abstract

Efficient and reproducible intracellular delivery is critical for manufacturing next generation cell therapies. Mechanoporation employs mechanical forces, including shear loading, adhesion, and compressive strain, to transiently permeabilize cell membranes and...

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Cite This Study

Gupta et al. (2026) studied this question.

synapsesocial.com/papers/6a06b983e7dec685947ac442 https://doi.org/10.1039/d6lc00143b

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A parametric study of mechanoporation through microfluidic design to modulate shear, compressive, and adhesion forces and loading rates

Key Points

Implication

Abstract

Discussion

Cite This Study

A parametric study of mechanoporation through microfluidic design to modulate shear, compressive, and adhesion forces and loading rates

Key Points

Implication

Abstract

Discussion

Cite This Study

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