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January 28, 2019Open Access

Cryopreservation of grapevine (Vitis spp.) shoot tips from growth chamber-sourced plants and histological observations

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Authors

JBJean Carlos BettoniOak Ridge Associated UniversitiesRBRemi BonnartCenter for Agricultural Resources ResearchASA. ShepherdUniversity of Stirling

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Implication

Randomized trial evaluates cryopreservation of shoot tips in Vitis plants, suggesting improved regrowth from non-in vitro sources.

Key Points

  • This research aims to evaluate the feasibility of using growth chamber-derived grapevine plants as a source for cryopreservation of shoot tips.
  • Utilized nodal sections from growth chamber plants for surface disinfection and tissue culture on pre-treatment medium for 2 weeks.
  • Performed preculture of shoot tips in a medium with sucrose and other additives before cryopreservation procedures using PVS2 at varying temperatures.
  • Conducted histological observations to assess meristem survival post-cryopreservation.
  • Cryopreserved shoot tips exhibited regrowth rates of 50% without and 55% with cold-acclimation after full-strength PVS2 treatment.
  • Histological analysis indicated survival of multiple meristems in cryopreserved shoot tips.
  • Successful cryopreservation was demonstrated in V. vinifera 'Chardonnay', 'Riesling', and V. hybrid 'Oppenheim' with favorable regrowth percentages.

Cite This Study

Bettoni et al. (2019) studied this question.

synapsesocial.com/papers/6a06e39a6b3d000707583cfehttps://doi.org/10.5073/vitis.2019.58.71-78
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