Triiodothyronine alleviated myocardial injury in sepsis-induced cardiomyopathy by inhibiting phospholamban, which also demonstrated excellent diagnostic performance for SIC with an AUC of 0.9503.
Does triiodothyronine alleviate myocardial injury in sepsis-induced cardiomyopathy?
Triiodothyronine protects against sepsis-induced cardiomyopathy by downregulating phospholamban, highlighting PLN as a potential diagnostic marker.
Effect estimate: AUC 0.9503 (95% CI 0.9014-0.9993)
models, respectively. Myocardial damage was detected via pathological tissue sections, a Cell Counting Kit-8 assay, an apoptosis assay and crystal violet staining. Intracellular calcium levels and reactive oxygen species were detected by Fluo‑4AM and DHE fluorescence. The protein and mRNA expression levels of JNK and c‑Jun were measured by western blotting and reverse transcription‑quantitative PCR to investigate the molecular mechanisms involved. Subsequently, 100 clinical patients were recruited to verify the clinical application value of PLN in SIC. The results revealed a significant negative correlation between PLN and T3 in the animal disease model. Furthermore, the expression levels of genes and proteins in the JNK/c‑Jun signaling pathway and PLN expression levels were decreased, whereas the expression levels of sarcoplasmic reticulum calcium ATPase were increased after T3 treatment. These results indicated that T3 alleviated myocardial injury in SIC by inhibiting PLN expression and its phosphorylation, which may be related to the JNK/c‑Jun signaling pathway. Accordingly, PLN may have clinical diagnostic value in patients with SIC.
Xie et al. (Thu,) conducted a other in Sepsis-induced cardiomyopathy (SIC) (n=86). Triiodothyronine (T3) vs. LPS (sepsis model) / Healthy controls was evaluated on Diagnostic performance of serum phospholamban (PLN) for SIC (AUC 0.9503, 95% CI 0.9014-0.9993). Triiodothyronine alleviated myocardial injury in sepsis-induced cardiomyopathy by inhibiting phospholamban, which also demonstrated excellent diagnostic performance for SIC with an AUC of 0.9503.