Key result
Systemic mapping details Cypher expression across developmental stages and adulthood in mice.
Why the study?
Conditional deletion or mutations in Cypher lead to human disease with unknown etiology and mechanism, prompting systematic investigation of Cypher expression across organs and developmental stages.
Mapping the expression pattern of Cypher in multiple organs provides a foundation for understanding its role in human diseases associated with Cypher mutations.
Answers are grounded in this paper and cite their sources.
Findings in mice should not yet inform clinical decisions; leaves open Cypher's roles in multi-organ human diseases.
Cypher, which is named ZASP/LDB3, one of the members of PDZ-LIM family, was well known to express in cardiac and skeletal muscles which played a functional role in muscles. It has shown that global deletion of Cypher in mice leads to lethality due to dysfunction of the skeletal muscles. Recently, the MGD database has been shown that conditional deletion of Cypher or gene mutations leads to human disease, the etiology and mechanism of which remained unknown. Therefore, it might be very important that we systemically investigated the expression of Cypher in organs or tissues from developmental stages through adulthood at first. We explored the expression pattern of Cypher by [Formula: see text]-gal staining of Cypher LacZ knockin mice combing with chemical staining, immunostaining and in situ hybridization, suggesting that Cypher might play a functional role in those organs. Further investigation will be done by cooperation with other groups by using the conditional deletion of Cypher or the point mutation of genes in the organs, which studied Cypher expression according to the MGD database.
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Fang et al. (2021) studied Cypher expression in mice. Cypher expression was systemically investigated in organs or tissues from developmental stages through adulthood in mice using beta-gal staining, immunostaining, and in situ hybridization.
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