Key result
Peristaltic pump and 3D-printed elements enable rapid isolation of murine heart cells for single-cell omics.
Why the study?
A protocol was needed to address the time required to extract cardiac cells, cell viability, and protocol scalability during cardiac cell isolation.
Provides a scalable and efficient protocol for the parallel isolation of viable cardiac cells from murine models, facilitating downstream bio-molecular analyses.
Facilitates efficient murine cardiac cell isolation for omics; leaves open human translation and clinical applicability.
This protocol features parallel isolation of myocytes and non-myocytes from murine hearts. It was designed with considerations for (1) time required to extract cardiac cells, (2) cell viability, and (3) protocol scalability. Here, a peristaltic pump and 3D-printed elements are combined to perfuse the heart with enzymes to dissociate cells. Myocytes and non-myocytes extracted using this protocol are separated by centrifugation and/or fluorescence-activated cell sorting for use in downstream applications including single-cell omics or other bio-molecular analyses. For complete details on the use and execution of this protocol, please refer to McLellan et al. (2020).
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Farrugia et al. (2021) studied this question. Protocol for parallel isolation of myocytes and non-myocytes was evaluated. A protocol combining a peristaltic pump and 3D-printed elements enables rapid, parallel isolation of myocytes and non-myocytes from murine hearts for downstream single-cell omics.
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