Identification and quantification of dandelion metabolites by NMR for the characterization and differentiation of dandelion germplasm

H NMR spectra, we identified and quantified 56 metabolites including nine compounds not previously reported in either Taraxacum species. Subsequently, we coupled the metabolite results with multivariate statistics, which reveal distinct metabolic fingerprints, highlighting variations within the dandelion species and plant organs. Organ type emerged as the primary determinant of metabolic variation, with species-specific differences most prominent in roots. This establishes baseline NMR profiles essential for rubber breeding biomarker discovery, underscoring NMR's utility in dandelion metabolomics and deepening understanding of their organ-specific metabolism.