Exposure of Cu and Mn to Cicer arietinum L. VAR. BGDâ€72 seeds induces morphological and biochemical changes in the plant

Copper (Cu) and Manganese (Mn) are micronutrients that are essential for normal growth and development of the plants. These transition metals are involved in cellular redox reactions. In the present study effect of various concentrations (Ck, 25, 50, 75, 100 and 125ppm) of Cu and Mn on the chlorophyll content, catalase (CAT) activity, malonedialdehyde (MDA) level, root biomass and tolerance index was investigated in 25 days old seedlings of Cicer arietinum L. Effect on morphological parameters such as seed germination and radicle length was investigated in 5 days old seedlings. At higher concentrations, Cu and Mn resulted in loss of the photosynthetic pigments (Chl a and Chl b). CAT activity significantly in root and shoot with the increase in Cu and Mn concentration as compared to control (Ck=untreated) plants. A similar trend was observed in MDA activity in leaves. Combination of Cu and Mn exhibited additive effect on these parameters.